Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells

Liu, Bo and Billington, Charlotte K. and Henry, Amanda P. and Bhaker, Sangita K. and Kheirallah, Alexander K. and Swan, Caroline and Hall, Ian P. (2018) Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells. Physiological Reports, 6 (2). e13508. ISSN 2051-817X

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Abstract

Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterise the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP-induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type.

Item Type: Article
Keywords: Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Respiratory Medicine
Identification Number: https://doi.org/10.14814/phy2.13508
Depositing User: Eprints, Support
Date Deposited: 15 Dec 2017 14:46
Last Modified: 02 Feb 2018 01:55
URI: http://eprints.nottingham.ac.uk/id/eprint/48775

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