The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway

Zhang, Xuebin and Jayaweera, Dasuni and Peters, Janny L. and Szecsi, Judit and Bendahmane, Mohammed and Roberts, Jeremy A. and Gonzalez-Carranza, Zinnia H. (2017) The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. PLoS ONE, 12 (12). e0189788/1-e0189788/17. ISSN 1932-6203

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Abstract

In Arabidopsis, the F-box HAWAIIAN SKIRT (HWS) protein is important for organ growth. Loss of function of HWS exhibits pleiotropic phenotypes including sepal fusion. To dissect the HWS role, we EMS-mutagenized hws-1 seeds and screened for mutations that suppress hws-1 associated phenotypes. We identified shs-2 and shs-3 (suppressor of hws-2 and 3) mutants in which the sepal fusion phenotype of hws-1 was suppressed. shs-2 and shs-3 (renamed hst-23/hws-1 and hst-24/hws-1) carry transition mutations that result in premature terminations in the plant homolog of Exportin-5 HASTY (HST), known to be important in miRNA biogenesis, function and transport. Genetic crosses between hws-1 and mutant lines for genes in the miRNA pathway, also suppress the phenotypes associated with HWS loss of function, corroborating epistatic relations between the miRNA pathway genes and HWS. In agreement with these data, accumulation of miRNA is modified in HWS loss or gain of function mutants. Our data propose HWS as a new player in the miRNA pathway, important for plant growth.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
Identification Number: 10.1371/journal.pone.0189788
Depositing User: Eprints, Support
Date Deposited: 11 Dec 2017 13:13
Last Modified: 18 Dec 2017 12:53
URI: http://eprints.nottingham.ac.uk/id/eprint/48648

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