Nitric oxide synthase and NAD(P)H oxidase modulate coronary endothelial cell growth
Bayraktutan, Ulvi (2004) Nitric oxide synthase and NAD(P)H oxidase modulate coronary endothelial cell growth. Journal of Molecular and Cellular Cardiology, 36 . pp. 277-286.
Reactive oxygen species (ROS) including nitric oxide (NO) and superoxide anion (O2-) are associated with cell migration, proliferation and many growth-related diseases. The objective of this study was to determine whether there was a reciprocal relationship between rat coronary microvascular endothelial cell (CMEC) growth and activity/expressions (mRNA and protein) of endothelial NO synthase (eNOS) and NAD(P)H oxidase enzymes. Proliferating namely, 50% confluent CMEC possessed approximately three-fold increased activity and expression of both enzymes compared to 100% confluent cells. Treatment of CMEC with an inhibitor of eNOS (L-NAME, 100M) increased cell proliferation as assessed via three independent methods i.e. cell counting, determination of total cellular protein levels and [3H]thymidine incorporation.
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