Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells

Vining, Kyle H. and Scherba, Jacob C. and Bever, Alaina and Alexander, Morgan R. and Celiz, Adam D. and Mooney, David J. (2017) Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells. Advanced Materials . ISSN 1521-4095

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Abstract

Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used. Based on these findings, thiol-ene chemistry is employed to achieve rapid light-curing and minimize residual monomer of the lead materials. Several triacrylate bulk polymers support DPSC adhesion, proliferation, and differentiation in vitro, and exhibit stiffness and tensile strength similar to existing dental materials. Conversely, materials composed of a trimethacrylate monomer or bisphenol A glycidyl methacrylate, which is a monomer standard in dental materials, do not support stem cell adhesion and negatively impact matrix and signaling pathways. Furthermore, thiol-ene polymerized triacrylates are used as permanent filling materials at the dentin-pulp interface in direct contact with irreversibly injured pulp tissue. These novel triacrylate-based biomaterials have potential to enable novel regenerative dental therapies in the clinic by both restoring teeth and providing a supportive niche for DPSCs.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: K. H. Vining, J. C. Scherba, A. M. Bever, M. R. Alexander, A. D. Celiz, D. J. Mooney, Adv. Mater. 2017, 1704486, which has been published in final form at http://dx.doi.org/10.1002/adma.201704486. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: dental materials; dental pulp stem cells; multi-functional acrylates, polymer microarray; differentiation
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1002/adma.201704486
Depositing User: Eprints, Support
Date Deposited: 06 Dec 2017 13:55
Last Modified: 14 Dec 2017 09:53
URI: http://eprints.nottingham.ac.uk/id/eprint/48580

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