Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach

Kostas, Emily Tsambika and Wilkinson, Stuart and White, Daniel A. and Cook, David J. (2017) Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach. Methods in Molecular Biology . ISSN 1940-6029

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Abstract

Accurate quantification of the carbohydrate content of biomass is crucial for many bio-refining processes. The most commonly followed protocol is typically a modification of the NREL-based assay (specifically designed for carbohydrate analysis from lignocellulosic biomass). However, this NREL protocol was revealed to be excessively thermochemically harsh for seaweed biomass. This can result in erroneously low total sugar quantification as the reaction severity can degrade a proportion of the liberated sugars to decomposition products such as furans. Here we describe an optimization of the total acid hydrolysis protocol for accurate quantification of the carbohydrate content of seaweeds. Different species of seaweed can be accurately evaluated for their carbohydrate contents by following this optimized method.

Item Type: Article
Additional Information: This is a post-peer-review, pre-copyedited version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/7651_2017_105
Keywords: Carbohydrate, Composition, Macroalgae, Mass balance, Optimization, Species
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Food Sciences
Identification Number: 10.1007/7651_2017_105
Depositing User: Kostas, Emily
Date Deposited: 05 Dec 2017 14:14
Last Modified: 07 Dec 2017 13:54
URI: http://eprints.nottingham.ac.uk/id/eprint/48494

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