Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

Grosse-Honebrink, Alexander and Schwarz, Katrin and Wang, Hengzheng and Minton, Nigel P. and Zhang, Ying (2017) Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants. Anaerobe, 48 . pp. 203-205. ISSN 1095-8274

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Abstract

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude.

Item Type: Article
Keywords: Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC)
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1016/j.anaerobe.2017.09.001
Depositing User: Eprints, Support
Date Deposited: 20 Oct 2017 08:42
Last Modified: 20 Oct 2017 11:35
URI: http://eprints.nottingham.ac.uk/id/eprint/47404

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