Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity

Pickles, S.F. and Pritchard, David I. (2017) Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Medical and Veterinary Entomology, 31 (2). pp. 200-206. ISSN 1365-2915

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Abstract

Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay.

Item Type: Article
Additional Information: This is the pre-peer reviewed version of the following article: PICKLES, S. F. and PRITCHARD, D. I. (2017), Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Med Vet Entomol, 31: 200–206. doi:10.1111/mve.12220, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1111/mve.12220
Depositing User: Pickles, Samantha
Date Deposited: 18 Oct 2017 08:34
Last Modified: 18 Oct 2017 08:37
URI: http://eprints.nottingham.ac.uk/id/eprint/47337

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