Embryo cell allocation patterns are not altered by biopsy but can be linked with further development

Sepulveda-Rincon, Lessly P. and Islam, Nadia and Marsters, Peter and Campbell, Bruce K. and Beaujean, Nathalie and Maalouf, Walid E. (2017) Embryo cell allocation patterns are not altered by biopsy but can be linked with further development. Reproduction . ISSN 1470-1626

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Abstract

It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2 cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo biopsy on cell allocation patterns during embryo pre-implantation in two different mouse strains and the effects of these patterns on further development. First, one blastomere of the 2-cell embryo was injected with a lipophilic tracer and cell allocation patterns were observed at blastocyst stage. Blastocysts were classified into orthogonal, deviant or random pattern. For the first experiment, embryos were biopsied at 8-cell stage and total cell counts (TCC) were annotated. Furthermore, non biopsied blastocysts were transferred into foster mothers. Then, pups and their organs were weighed two weeks after birth. Random pattern was significantly recurrent (≈60%), against orthogonal (<22%) and deviant (<22%) patterns among groups. These patterns were not affected by biopsy procedure. However, TCC on deviant embryos were reduced after biopsy. Moreover, no differences were found between patterns for implantation rates, litter size, live offspring and organ weights (lungs, liver, pancreas and spleen). However, deviant pups presented heavier hearts and orthogonal pups presented lighter kidneys among the group. In conclusion, these results suggest that single blastomere removal does not disturb cell allocation patterns during pre-implantation. Nonetheless, the results suggest that embryos following different cell allocation patterns present different coping mechanisms against in-vitro manipulations and further development might be altered.

Item Type: Article
Additional Information: This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://www.reproduction-online.org/content/early/2017/09/29/REP-17-0514
Keywords: Embryo biopsy, pre-patterning, PGD/PGS, organ development, embryo transfer, implantation.
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number: 10.1530/REP-17-0514
Depositing User: Maalouf, Walid E
Date Deposited: 13 Oct 2017 10:36
Last Modified: 13 Oct 2017 17:49
URI: http://eprints.nottingham.ac.uk/id/eprint/47235

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