A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering

Boukari, Yamina and Qutachi, Omar and Morris, Andrew P. and Doughty, Stephen W. and Billa, Nashiru (2017) A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering. Journal of Biomaterials Science, Polymer Edition . ISSN 0920-5063

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Abstract

The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37°C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p < 0.05). The formulation also sintered at 37°C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37°C in situ.

Item Type: Article
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition on 17 August 2017, available online: http://www.tandfonline.com/10.1080/09205063.2017.1364100.
Keywords: polymeric biomaterials, controlled delivery, poly (lactic-co-glycolic acid) (PLGA), microspheres, protein delivery, tissue engineering, mechanical properties, formulation
Schools/Departments: University of Nottingham, Malaysia > Faculty of Science > School of Pharmacy
University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1080/09205063.2017.1364100
Depositing User: BILLA, NASHIRU
Date Deposited: 11 Oct 2017 09:29
Last Modified: 13 Oct 2017 05:48
URI: http://eprints.nottingham.ac.uk/id/eprint/47107

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