Maternal dexamethasone treatment alters tissue and circulating components of the renin-angiotensin system in the pregnant ewe and fetus

Forhead, Alison J. and Jellyman, Juanita K. and De Blasio, Miles J. and Johnson, Emma and Giussani, Dino A. and Broughton Pipkin, Fiona and Fowden, Abigail L. (2015) Maternal dexamethasone treatment alters tissue and circulating components of the renin-angiotensin system in the pregnant ewe and fetus. Endocrinology, 156 (8). pp. 3038-3046. ISSN 1945-7170

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Abstract

Antenatal synthetic glucocorticoids promote fetal maturation in pregnant women at risk of preterm delivery and their mechanism of action may involve other endocrine systems. This study investigated the effect of maternal dexamethasone treatment, at clinically relevant doses, on components of the renin-angiotensin system (RAS) in the pregnanteweandfetus.From125 days of gestation (term, 145_2 d), 10 ewes carrying single fetuses of mixed sex (3 female, 7 male) were injected twice im, at 10–11 PM, with dexamethasone (2_12 mg, n_5) or saline (n_5) at 24-hour intervals. At 10 hours after the second injection, maternal dexamethasone treatment increased angiotensin-converting enzyme (ACE) mRNA levels in the fetal lungs, kidneys, and heart and ACE concentration in the circulation and lungs, but not kidneys, of the fetuses. Fetal cardiac mRNA abundance of angiotensin II (AII) type 2 receptor decreased after maternal dexamethasone treatment. Between the two groups of fetuses, there were no significant differences in plasma angiotensinogen or renin concentrations; in transcript levels of renal renin, or AIItype 1 or 2 receptors in the lungs and kidneys; or in pulmonary, renal or cardiac protein content of the AII receptors. In the pregnant ewes, dexamethasone administration increased pulmonary ACE and plasma angiotensinogen, and decreased plasma renin, concentrations. Some of the effects of dexamethasone treatment on the maternal and fetal RAS were associated with altered insulin and thyroid hormone activity. Changes in the local and circulating RAS induced by dexamethasone exposure in utero may contribute to the maturational and tissue-specific actions of antenatal glucocorticoid treatment.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number: 10.1210/en.2015-1197
Depositing User: Broughton_Pipkin, Professor Fiona
Date Deposited: 05 Oct 2017 08:58
Last Modified: 13 Oct 2017 06:11
URI: http://eprints.nottingham.ac.uk/id/eprint/47009

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