Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes

Hdud, Ismail M. and Mobasheri, Ali and Loughna, Paul (2014) Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes. SpringerPlus, 3 . 59/1-59/3. ISSN 2193-1801

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Abstract

Background

Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels.

Findings

Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected.

Conclusions

Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca2+.

Item Type: Article
Keywords: Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1186/2193-1801-3-59
Depositing User: Loughna, Paul
Date Deposited: 25 Sep 2017 11:09
Last Modified: 14 Oct 2017 08:27
URI: http://eprints.nottingham.ac.uk/id/eprint/46612

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