Extracellular matrix-derived hydrogels for dental stem cell delivery

Viswanath, Aiswarya and Vanacker, Julie and Germain, Loic and Leprince, Julien G. and Diogenes, Anibal and Shakesheff, Kevin M. and White, Lisa J. and des Rieux, Anne (2016) Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of Biomedical Materials Research Part A, 105 (1). pp. 319-328. ISSN 1552-4965

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Abstract

Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord and dentine ECM hydrogels exhibited distinct structural, mechanical and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behaviour for constructive spinal cord regeneration

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Viswanath A, Vanacker J, Germain L, Leprince JG, Diogenes A, Shakesheff KM, White LJ, des Rieux A. 2017. Extracellular matrix-derived hydrogels for dental stem cell delivery. J Biomed Mater Res Part A 2017:105A:319–328, which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35901. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: ECM hydrogels, Dental stem cell delivery, Spinal cord, Bone, Dentine
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1002/jbm.a.35901
Depositing User: White, Lisa
Date Deposited: 15 Sep 2017 09:54
Last Modified: 15 Sep 2017 10:00
URI: http://eprints.nottingham.ac.uk/id/eprint/46382

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