Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge

Clementi, Nicola and Criscuolo, Elena and Cappelletti, Francesca and Quaranta, Paola and Pistello, Mauro and Diotti, Roberta A. and Sautto, Giuseppe A. and Tarr, Alexander W. and Mailland, Federico and Concas, Daniela and Burioni, Roberto and Clementi, Massimo and Mancini, Nicasio (2017) Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge. Antiviral Research, 143 . pp. 48-61. ISSN 1872-9096

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Abstract

The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission.

Item Type: Article
Keywords: HSV disseminated infection; Cell-to-cell virus transmission; Human antibodies; In vivo protection
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1016/j.antiviral.2017.03.028
Depositing User: Eprints, Support
Date Deposited: 13 Sep 2017 14:56
Last Modified: 15 Oct 2017 04:49
URI: http://eprints.nottingham.ac.uk/id/eprint/46065

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