Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media

Zhang, Qimei and Morgan, Stephen P. and Mather, Melissa L. (2017) Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media. Small, 13 (33). 1602895/1-1602895/12. ISSN 1613-6829

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Abstract

A new approach to fluorescence imaging in optically turbid media centered on the use of nanoscale ultrasound-switchable FRET based liposome contrast agents is reported. Liposomes containing lipophilic carbocyanine dyes as FRET pairs with emission wavelengths located in the near-infrared window were prepared. The efficacy of FRET and self-quenching for liposomes with a range of fluorophore concentrations was first calculated from measurement of the liposome emission spectra. Exposure of the liposomes to ultrasound resulted in changes in the detected fluorescent signal, the nature of which depending on the fluorophores used, detection wavelength and the fluorophore concentration. Line scanning of a tube containing the contrast agents with 1 mm inner diameter buried at a depth of 1 cm in a heavily scattering tissue phantom demonstrated an improvement in image spatial resolution by a factor of 6.3 as compared with images obtained in the absence of ultrasound. Improvements were also seen in image contrast with the highest obtained being 9% for a liposome system containing FRET pairs. Overall the results obtained provide evidence of the potential the nanoscale ultrasound-switchable FRET based liposomes studied here have for in vivo fluorescence imaging.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media. Qimei Zhang, Stephen P. Morgan, and Melissa L. Mather, Small, 13 (2017), 1602895 which has been published in final form at doi:10.1002/smll.201602895. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: FRET; liposome; nanoscale; near-infrared; ultrasound switchable fluorescence tomography
Schools/Departments: University of Nottingham, UK > Faculty of Engineering > Department of Electrical and Electronic Engineering
Identification Number: 10.1002/smll.201602895
Depositing User: Zhang, Qimei
Date Deposited: 15 Sep 2017 10:26
Last Modified: 16 Oct 2017 18:04
URI: http://eprints.nottingham.ac.uk/id/eprint/45836

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