Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorous predation

Kuru, Erkin and Lambert, Carey and Jonathon, Rittichier and Till, Rob and Ducret, Adrien and Derouaux, Adeline and Gray, Joe and Biboy, Jacob and Vollmer, Waldemar and VanNieuwenhze, Michael and Brun, Yves and Sockett, Liz (2017) Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorous predation. Nature Microbiology . ISSN 2058-5276

[img] PDF - Repository staff only until 3 April 2018. - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (16MB)

Abstract

Modification of essential bacterial peptidoglycan (PG) containing cell walls can lead to antibiotic resistance, for example β-lactam resistance by L,D-transpeptidase activities. Predatory Bdellovibrio bacteriovorus are naturally antibacterial and combat infections by traversing, modifying and finally destroying walls of Gram-negative prey bacteria, modifying their own PG as they grow inside prey. Historically, these multi-enzymatic processes on two similar PG walls have proved challenging to elucidate. Here, with a PG labelling approach utilizing timed pulses of multiple fluorescent D-amino acids (FDAAs), we illuminate dynamic changes that predator and prey walls go through during the different phases of bacteria:bacteria invasion. We show formation of a reinforced circular port-hole in the prey wall; L,D-transpeptidaseBd mediated D-amino acid modifications strengthening prey PG during Bdellovibrio invasion and a zonal mode of predator-elongation. This process is followed by unconventional, multi-point and synchronous septation of the intracellular Bdellovibrio, accommodating odd- and even-numbered progeny formation by non-binary division.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1038/s41564-017-0029-y
Depositing User: Lambert, Carey
Date Deposited: 07 Sep 2017 13:40
Last Modified: 13 Oct 2017 16:37
URI: http://eprints.nottingham.ac.uk/id/eprint/45493

Actions (Archive Staff Only)

Edit View Edit View