Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells

Xu, Sha-Sha and Liu, Qing-Mei and Xiao, An-Feng and Maleki, Soheila J. and Alcocer, Marcos and Gao, Yuan-Yuan and Cao, Min-Jie and Liu, Guang-Ming (2017) Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells. Journal of Agricultral and Food Chemistry, 65 (15). pp. 3212-3222. ISSN 1520-5118

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Abstract

In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2−97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease.

Item Type: Article
Keywords: Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1021/acs.jafc.7b00389
Depositing User: Eprints, Support
Date Deposited: 06 Sep 2017 07:54
Last Modified: 16 Oct 2017 22:51
URI: http://eprints.nottingham.ac.uk/id/eprint/45461

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