Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model

Geng, Shizhong and Tian, Qin and Guo, Rongxian and Jiao, Yang and Barrow, Paul and Yin, Chao and Wang, Yaonan and Geng, Haopeng and Pan, Zhiming and Jiao, Xinan (2017) Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model. Microbial Pathogenesis, 104 . pp. 146-150. ISSN 1096-1208

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Abstract

A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation.

Item Type: Article
Keywords: Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1016/j.micpath.2017.01.014
Depositing User: Eprints, Support
Date Deposited: 23 Aug 2017 10:49
Last Modified: 23 Aug 2017 11:00
URI: http://eprints.nottingham.ac.uk/id/eprint/45099

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