Formation of the stomatal outer cuticular ledge requires a guard cell wall proline-rich protein

Hunt, Lee and Amsbury, Samuel and Baillie, Alice and Movahedi, Mahsa and Mitchell, Alice and Afsharinafar, Mana and Swarup, Kamal and Denyer, Thomas and Hobbs, Jamie K. and Swarup, Ranjan and Fleming, Andrew J. and Gray, Julie E. (2017) Formation of the stomatal outer cuticular ledge requires a guard cell wall proline-rich protein. Plant Physiology, 174 (2). pp. 689-699. ISSN 1532-2548

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Abstract

Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to withstand the large increases in turgor pressure that have to be generated to open the pore that they surround. We have characterised FOCL1, a guard cell-expressed, secreted protein with homology to hydroxyproline-rich cell wall proteins. FOCL1-GFP localises to the guard cell outer cuticular ledge and plants lacking FOCL1 produce stomata without a cuticular ledge. Instead the majority of stomatal pores are entirely covered-over by a continuous fusion of the cuticle, and consequently plants have decreased levels of transpiration and display drought tolerance. The focl1 guard cells are larger and less able to reduce the aperture of their stomatal pore in response to closure signals suggesting that the flexibility of guard cell walls is impaired. FOCL1 is also expressed in lateral root initials where it aids lateral root emergence. We propose that FOCL1 acts in these highly specialised cells of the stomata and root to impart cell wall strength at high turgor and/or to facilitate interactions between the cell wall and the cuticle.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
Identification Number: 10.1104/pp.16.01715
Depositing User: Eprints, Support
Date Deposited: 15 Aug 2017 10:26
Last Modified: 18 Oct 2017 17:50
URI: http://eprints.nottingham.ac.uk/id/eprint/44897

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