Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells

Gomzikova, Marina and Zhuravleva, Margarita and Miftakhova, Regina and Arkhipova, Svetlana and Evtugin, Vladimir and Khaiboullina, Sventlana and Kiyasov, Andrey and Persson, Jenny and Mongan, Nigel P. and Pestell, Richard and Rizvanov, Albert A. (2017) Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells. Oncotarget, 8 (41). pp. 70496-70507. ISSN 1949-2553

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Abstract

Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.

Item Type: Article
Keywords: extracellular vesicles, membrane vesicles, Cytochalasin B-induced membrane vesicles, angiogenesis, cell-free therapy
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.18632/oncotarget.19723
Depositing User: Mongan, Nigel
Date Deposited: 04 Aug 2017 12:22
Last Modified: 25 Oct 2017 09:42
URI: http://eprints.nottingham.ac.uk/id/eprint/44621

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