An in vitro model for assessing effective scrapie decontamination

Gough, Kevin C. and Baker, C.A. and Maddison, B.C. (2017) An in vitro model for assessing effective scrapie decontamination. Veterinary Microbiology, 207 . pp. 138-142. ISSN 1873-2542

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Abstract

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity within the farm is not adequately removed by current recommended guidelines for farm decontamination. We describe an in vitro method for modelling decontamination, specifically the removal of scrapie prions from the surface of concrete fomites within buildings that have housed scrapie infected animals. Concrete that had been spiked with low amounts of a diluted scrapie positive brain homogenate was sampled before and after decontamination. Extracts were used to seed a semi-quantitative serial protein misfolding cyclic amplification assay (sPMCA). We demonstrate that methods currently recommended for prion decontamination result in inadequate reduction of prion seeding activity within this in vitro assay. Effective treatment was achieved using repeat dosing of surfaces with 20,000 ppm available chlorine for 4 h.

Item Type: Article
Keywords: Scrapie; Prion; sPMCA; Decontamination; Fomite
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1016/j.vetmic.2017.05.018
Depositing User: Eprints, Support
Date Deposited: 27 Jul 2017 10:53
Last Modified: 08 Dec 2017 19:54
URI: http://eprints.nottingham.ac.uk/id/eprint/44469

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