Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia

Mistry, Hiten D. and Kurlak, L.O. and Mansour, Yosef and Zurkinden, Line and Mohaupt, Markus and Escher, Genevieve (2017) Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia. Journal of Lipid Research, 58 . pp. 1186-1185. ISSN 1539-7262

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Abstract

Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P < 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis.

Item Type: Article
Keywords: lipid/efflux; 27-hydroxycholesterol; apolipoproteins; pregnancy; hypertension; sterol 27-hydroxylase
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number: 10.1194/jlr.M071985
Depositing User: Mistry, Hiten
Date Deposited: 21 Jul 2017 11:07
Last Modified: 12 Oct 2017 23:32
URI: http://eprints.nottingham.ac.uk/id/eprint/44336

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