Urine protein concentration estimation for biomarker discovery

Mistry, Hiten D. and Bramham, Kate and Weston, Andrew and Ward, Malcolm and Thompson, Andrew and Chappell, Lucy C. (2013) Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3 (4). pp. 211-214. ISSN 2210-7797

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Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Item Type: Article
Keywords: Protein concentration assays; Proteomics; Urine
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number: https://doi.org/10.1016/j.preghy.2013.07.001
Depositing User: Mistry, Hiten
Date Deposited: 21 Jul 2017 09:47
Last Modified: 12 Oct 2017 23:32
URI: http://eprints.nottingham.ac.uk/id/eprint/44323

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