Rapeseed napin and cruciferin are readily digested by poultry

Kasprzak, M.M. and Houdijk, J.G.M. and Liddell, S. and Davis, K. and Olukosi, O.A. and Kightley, S. and White, G.A. and Wiseman, J. (2017) Rapeseed napin and cruciferin are readily digested by poultry. Journal of Animal Physiology and Animal Nutrition, 101 (4). pp. 658-666. ISSN 1439-0396

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Abstract

Rapeseed proteins have been considered as being poorly digestible in the gut of non-ruminants. The aim of the study was to assess the digestibility of napin and cruciferin in ileal digesta of broiler chickens, testing sixteen samples of rapeseed co-products with protein levels ranging from 293 g/kg to 560 g/kg dry matter. Each sample was included into a semi-synthetic diet at a rate of 500 g/kg and evaluated with broiler chickens in a randomised design. Dietary and ileal digesta proteins were extracted and identified by gel-based liquid chromatography–tandem mass spectrometry (LC-MS/MS). Three isomers of napin (a 2S albumin) and nine cruciferins (an 11S globulin) were identified in the rapeseed co-products, whereas six endogenous enzymes such as trypsin (I-P1, II-P29), chymotrypsin (elastase and precursor), carboxypeptidase B and α-amylase were found in the ileal digesta. It is concluded that as none of the rapeseed proteins were detected in the ileal digesta, rapeseed proteins can be readily digested by broiler chickens, irrespective of the protein content in the diet.

Item Type: Article
Additional Information: This is the pre-peer reviewed version of the following article: Kasprzak, M.M., Houdijk, J.G.M., Liddell, S., Davis, K., Olukosi, O.A., Kightley, S., White, G.A. and Wiseman, J. (2017), Rapeseed napin and cruciferin are readily digested by poultry. J Anim Physiol Anim Nutr, 101: 658–666, which has been published in final form at doi:10.1111/jpn.12576. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: napin; cruciferin; protein; rapeseed meal; rapeseed cake; chickens.
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1111/jpn.12576
Depositing User: Eprints, Support
Date Deposited: 18 Jul 2017 08:15
Last Modified: 12 Oct 2017 23:06
URI: http://eprints.nottingham.ac.uk/id/eprint/44248

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