The impact of detergents on the tissue decellularization process: a ToF-SIMS study

White, Lisa J., Taylor, Adam J., Faulk, Denver M., Keane, Timothy J., Saldin, Lindsey T., Reing, Janet E., Swinehart, Ilea T., Turner, Neill J., Ratner, Buddy D. and Badylak, Stephen F. (2017) The impact of detergents on the tissue decellularization process: a ToF-SIMS study. Acta Biomaterialia, 50 . pp. 207-219. ISSN 1878-7568

Full text not available from this repository.


Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface ligand landscape remains a considerable challenge. Here we describe the use of time of flight secondary ion mass spectroscopy (ToF-SIMS) to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds. We detected residual detergent fragments, specifically from Triton X-100, sodium deoxycholate and sodium dodecyl sulphate (SDS) in decellularized scaffolds; increased SDS concentrations from 0.1% to 1.0% increased both the intensity of SDS fragments and adverse cell outcomes. We also identified cellular remnants, by detecting phosphate and phosphocholine ions in PAA and CHAPS decellularized scaffolds. The present study demonstrates ToF-SIMS is not only a powerful tool for characterization of biologic scaffold surface molecular functionality, but also enables sensitive assessment of decellularization efficacy.

Item Type: Article
Keywords: extracellular matrix, biologic scaffold, decellularization, ToF-SIMS, detergents
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number:
Depositing User: White, Lisa
Date Deposited: 17 Jul 2017 10:41
Last Modified: 04 May 2020 18:32

Actions (Archive Staff Only)

Edit View Edit View