The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses

Gao, Huijie and Sun, Yipeng and Hu, Jiao and Qi, Lu and Wang, Jinliang and Xiong, Xin and Wang, Yu and He, Qiming and Lin, Yang and Kong, Weili and Seng, Lai-Giea and Sun, Honglei and Pu, Juan and Chang, Kin-Chow and Liu, Xiufan and Liu, Jinhua (2015) The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses. Scientific Reports, 5 (1). p. 8262. ISSN 2045-2322

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Abstract

PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this study, we report on the pathogenicity and pathological effects of PA-X deficient 2009 pandemic H1N1 (pH1N1) and highly pathogenic avian influenza H5N1 viruses. We found that loss of PA-X expression in pH1N1 and H5N1 viruses increased viral replication and apoptosis in A549 cells and increased virulence and host inflammatory response in mice. In addition, PA-X deficient pH1N1 and H5N1 viruses up-regulated PA mRNA and protein synthesis and increased viral polymerase activity. Loss of PA-X was also accompanied by accelerated nuclear accumulation of PA protein and reduced suppression of PA on non-viral protein expression. Our study highlights the effects of PA-X on the moderation of viral pathogenesis and pathogenicity.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1038/srep08262
Depositing User: Eprints, Support
Date Deposited: 05 Jul 2017 10:53
Last Modified: 06 Jul 2017 14:28
URI: http://eprints.nottingham.ac.uk/id/eprint/43991

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