Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging

Mollink, Jeroen and Kleinnijenhuis, Michiel and Cappellen van Walsum, Anne-Marie van and Sotiropoulos, Stamatios N. and Cottaar, Michiel and Mirfin, Christopher and Heinrich, Mattias P. and Jenkinson, Mark and Pallebage-Gamarallage, Menuka and Ansorge, Olaf and Jbabdi, Saad and Miller, Karla L. (2017) Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging. NeuroImage, 157 . pp. 561-574. ISSN 1095-9572

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (4MB) | Preview

Abstract

Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain’s fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology.

Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract.

The region-wise analysis yielded correlation coefficients of r=0.79 for the diffusion MRI and histology comparison, while r=0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI.

These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data.

Item Type: Article
Keywords: Dispersion; Diffusion MRI; Post-mortem; Polarized Light Imaging; Myelin; Astrocytes; Validation
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Physics and Astronomy
Identification Number: 10.1016/j.neuroimage.2017.06.001
Depositing User: Eprints, Support
Date Deposited: 14 Jun 2017 10:29
Last Modified: 11 Jul 2017 14:23
URI: http://eprints.nottingham.ac.uk/id/eprint/43569

Actions (Archive Staff Only)

Edit View Edit View