Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea

Nwidu, L.L. and Elmorsy, Ekramy and Thornton, Jack and Wijamunige, Buddhika and Wijesekara, Anusha and Tarbox, Rebecca and Warren, Averil and Carter, Wayne G. (2017) Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea. Pharmaceutical Biology . ISSN 1744-5116 (In Press)

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Abstract

Context: There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).

Objective: To quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities, and cell cytotoxicity.

Materials and methods: Plant stem, leaves, and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200 μg//mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid content were determined, and cytotoxicity evaluated using human hepatocytes.

Results: C. lutea exhibited concentration dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀ = 140 μg/mL); for the leaves the chloroform leaf fraction (IC₅₀ = 60 μg/mL/mL); and for roots, the methanol, ethyl acetate, and aqueous root fractions (IC₅₀ = 0.3-3 μg/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf, or root concentration. Total phenolics were highest in the stem: ~632 mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was highest in the leaves: ~297 mg quercetin equivalents/g for a chloroform leaf fraction. At 1 μg/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes.

Discussion and conclusion: C. lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer’s and other diseases characterized by a cholinergic deficit.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine
Depositing User: Eprints, Support
Date Deposited: 13 Jun 2017 09:51
Last Modified: 18 Oct 2017 17:38
URI: http://eprints.nottingham.ac.uk/id/eprint/43529

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