RECQL4 helicase has oncogenic potential in sporadic breast cancers

Arora, Arvind and Agarwal, Devika and Abdel-Fatah, Tarek M.A. and Lu, Huiming and Croteau, Deborah L. and Moseley, Paul and Aleskandarany, Mohammed A. and Green, Andrew R. and Ball, Graham and Rakha, Emad A. and Chan, Stephen Y.T. and Ellis, Ian O. and Wang, Lisa L. and Zhao, Yongliang and Balajee, Adayabalam S. and Bohr, Vilhelm A. and Madhusudan, Srinivasan (2016) RECQL4 helicase has oncogenic potential in sporadic breast cancers. Journal of Pathology, 238 (4). pp. 495-501. ISSN 1096-9896

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Abstract

RECQL4 helicase is a molecular motor that unwinds DNA, a process essential during DNA replication and DNA repair. Germ-line mutations in RECQL4 cause type II Rothmund–Thomson syndrome (RTS), characterized by a premature ageing phenotype and cancer predisposition. RECQL4 is widely considered to be a tumour suppressor, although its role in human breast cancer is largely unknown. As the RECQL4 gene is localized to chromosome 8q24, a site frequently amplified in sporadic breast cancers, we hypothesized that it may play an oncogenic role in breast tumourigenesis. To address this, we analysed large cohorts for gene copy number changes (n = 1977), mRNA expression (n = 1977) and protein level (n = 1902). Breast cancer incidence was also explored in 58 patients with type II RTS. DNA replication dynamics and chemosensitivity was evaluated in RECQL4-depleted breast cancer cells in vitro. Amplification or gain in gene copy number (30.6%), high-level mRNA expression (51%) and high levels of protein (23%) significantly associated with aggressive tumour behaviour, including lymph node positivity, larger tumour size, HER2 overexpression, ER-negativity, triple-negative phenotypes and poor survival. RECQL4 depletion impaired the DNA replication rate and increased chemosensitivity in cultured breast cancer cells. Thus, although recognized as a ’safe guardian of the genome’, our data provide compelling evidence that RECQL4 is tumour promoting in established breast cancers.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Arora, A., Agarwal, D., Abdel-Fatah, T.M., Lu, H., Croteau, D.L., Moseley, P., Aleskandarany, M.A., Green, A.R., Ball, G., Rakha, E.A., Chan, S.Y., Ellis, I.O., Wang, L.L., Zhao, Y., Balajee, A.S., Bohr, V.A. and Madhusudan, S. (2016), RECQL4 helicase has oncogenic potential in sporadic breast cancers. J. Pathol., 238: 495–501, which has been published in final form at doi:10.1002/path.4681. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: RECQL4 helicase; breast cancer; tumour suppressor; oncogene
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Cancer and Stem Cells
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine
Identification Number: 10.1002/path.4681
Depositing User: Madhusudan, Srinivasan
Date Deposited: 23 May 2017 14:37
Last Modified: 24 May 2017 13:03
URI: http://eprints.nottingham.ac.uk/id/eprint/43062

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