Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

Růžička, Kamil and Zhang, Mi and Campilho, Ana and Bodi, Zsuzsanna and Kashif, Muhammad and Saleh, Mária and Eeckhout, Dominique and El-Showk, Sedeer and Li, Hongying and Zhong, Silin and Jaeger, Geert De and Mongan, Nigel P. and Hejátko, Jan and Helariutta, Ykä and Fray, Rupert G. (2017) Identification of factors required for m6A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. New Phytologist . ISSN 1469-8137

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Abstract

N6-adenosine methylation (m6A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood.

Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6A writer proteins in Arabidopsis thaliana.

The components required for m6A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6A methylation plays a role in developmental decisions during pattern formation.

The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m6A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.

Item Type: Article
Keywords: Arabidopsis, HAKAI, mRNA methylation, N6-adenosine methylation (m6A), Protoxylem, VIRILIZER
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1111/nph.14586
Depositing User: Eprints, Support
Date Deposited: 19 May 2017 13:22
Last Modified: 19 May 2017 19:34
URI: http://eprints.nottingham.ac.uk/id/eprint/42964

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