Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile

van Eijk, Erika and Paschalis, Vasileios and Green, Matthew and Friggen, Annemieke H. and Larson, Marilynn A. and Spriggs, Keith and Briggs, Geoffrey S. and Soultanas, Panos and Smits, Wiep Klaas (2016) Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile. Open Biology, 6 (12). ISSN 2046-2441

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Abstract

DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA replication in pathogenic microorganisms. Clostridium difficile is the main cause of healthcare-associated diarrhoea and its DNA replication machinery is virtually uncharacterized. We identify and characterize the mechanistic details of the putative replicative helicase (CD3657), helicase-loader ATPase (CD3654) and primase (CD1454) of C. difficile, and reconstitute helicase and primase activities in vitro We demonstrate a direct and ATP-dependent interaction between the helicase loader and the helicase. Furthermore, we find that helicase activity is dependent on the presence of primase in vitro The inherent trinucleotide specificity of primase is determined by a single lysine residue and is similar to the primase of the extreme thermophile Aquifex aeolicus. However, the presence of helicase allows more efficient de novo synthesis of RNA primers from non-preferred trinucleotides. Thus, loader-helicase-primase interactions, which crucially mediate helicase loading and activation during DNA replication in all organisms, differ critically in C. difficile from that of the well-studied Gram-positive Bacillus subtilis model.

Item Type: Article
Keywords: DNA replication initiation, helicase loading and activation, primase trinucleotide specificity, ATPase, Clostridium difficile
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Chemistry
University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1098/rsob.160272
Depositing User: Spriggs, Keith
Date Deposited: 17 May 2017 11:54
Last Modified: 17 May 2017 16:18
URI: http://eprints.nottingham.ac.uk/id/eprint/42874

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