A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology

Gillis, Richard B. and Rowe, Arthur J. and Adams, Gary G. and Harding, Stephen E. (2015) A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30 (2). pp. 142-157. ISSN 0264-8725

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Abstract

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.

Item Type: Article
Keywords: analytical ultracentrifugation, SEC-MALS, viscometry, light scattering, hydrodynamics, biopolymer, polydisperse
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1080/02648725.2014.994870
Depositing User: Eprints, Support
Date Deposited: 11 May 2017 10:56
Last Modified: 12 May 2017 18:55
URI: http://eprints.nottingham.ac.uk/id/eprint/42765

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