On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review

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Morris, Gordon A., Adams, Gary G. and Harding, Stephen E. (2014) On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review. Food Hydrocolloids, 42 . pp. 318-334. ISSN 0268-005X

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Abstract

Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance.

Discussion: Here the use of hydrodynamic tools such as sedimentation velocity, sedimentation equilibrium, size exclusion chromatography e multi-angle light scattering (SEC-MALS), and viscometry are considered highlighting some recent developments in methodology and the application of these to help better understand polysaccharide structureefunction relationships.

Conclusions: The size and shape of polysaccharides in solution can be estimated in a variety of ways. Molar masses and heterogeneities can be estimated to a good precision by sedimentation velocity, sedimentation equilibrium and SEC-MALS. An approximate idea of conformation and flexibility can be obtained from power-law coefficients and the Wales van Holde parameter. More sophisticated estimates can be obtained by combining methods together to yield the persistence length.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/740882
Keywords: Polysaccharides ; Size ; Conformation ; Structure-function relationships
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1016/j.foodhyd.2014.04.014
Depositing User: Eprints, Support
Date Deposited: 11 May 2017 10:22
Last Modified: 04 May 2020 16:58
URI: https://eprints.nottingham.ac.uk/id/eprint/42756

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