Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins

Harding, Stephen E. and Adams, Gary G. and Almutairi, Fahad and Alzahrani, Qushmua and Erten, Tayyibe and Samil Kök, M. and Gillis, Richard B. (2015) Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins. In: Analytical Ultracentrifugation. Methods in Enzymology (562). Academic Press, pp. 391-439. ISBN 9780128029084

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Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered.

Item Type: Book Section
Additional Information: Series editor, James Cole.
Keywords: Size; Shape; Interactions; SEDFIT-MSTAR; Extended Fujita; Flexibility
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Health Sciences
University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1016/bs.mie.2015.06.043
Depositing User: Eprints, Support
Date Deposited: 04 Apr 2017 08:29
Last Modified: 02 Jul 2018 09:12
URI: http://eprints.nottingham.ac.uk/id/eprint/41733

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