Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods

Harding, Stephen E. and Abdelhameed, Ali Saber and Gillis, Richard B. and Morris, Gordon A. and Adams, Gary G. (2015) Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In: Carbohydrate-based vaccines. Methods in molecular biology (1331). Springer, pp. 211-227.

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Abstract

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.

Item Type: Book Section
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://link.springer.com/protocol/10.1007%2F978-1-4939-2874-3_13
Depositing User: Eprints, Support
Date Deposited: 03 Apr 2017 14:04
Last Modified: 04 Apr 2017 09:15
URI: http://eprints.nottingham.ac.uk/id/eprint/41724

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