Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans

Gillis, Richard B. and Adams, Gary G. and Besong, David T.M. and Machová, Eva and Ebringerová, Anna and Rowe, Arthur J. and Harding, Stephen E. and Patel, Trushar R. (2017) Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans. European Biophysics Journal, 46 (3). pp. 235-245. ISSN 0175-7571

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Abstract

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins).

Item Type: Article
Keywords: AUC; Extended Fujita approach; MULTISIG; SEDFIT-MSTAR; Sedimentation
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences
University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1007/s00249-016-1159-5
Depositing User: Eprints, Support
Date Deposited: 03 Apr 2017 08:22
Last Modified: 04 Apr 2017 11:44
URI: http://eprints.nottingham.ac.uk/id/eprint/41686

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