Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat

Atkinson, Jonathan A. and Wingen, Luzie U. and Griffiths, Marcus and Pound, Michael P. and Gaju, Oorbessy and Foulkes, John and Le Gouis, Jacques and Griffiths, Simon and Bennett, Malcolm J. and King, Julie and Wells, Darren M. (2015) Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat. Journal of Experimental Botany, 66 (8). pp. 2283-2292. ISSN 1460-2431

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Abstract

Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. A root phenotyping pipeline, consisting of a germination paper-based screen combined with image segmentation and analysis software, was developed and used to characterize seedling traits in 94 doubled haploid progeny derived from a cross between the winter wheat cultivars Rialto and Savannah. Field experiments were conducted to measure mature plant height, grain yield, and nitrogen (N) uptake in three sites over 2 years. In total, 29 quantitative trait loci (QTLs) for seedling root traits were identified. Two QTLs for grain yield and N uptake co-localize with root QTLs on chromosomes 2B and 7D, respectively. Of the 29 root QTLs identified, 11 were found to co-localize on 6D, with four of these achieving highly significant logarithm of odds scores (>20). These results suggest the presence of a major-effect gene regulating seedling root vigour/growth on chromosome 6D.

Item Type: Article
Keywords: high-throughput phenotyping, root system architecture
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
University of Nottingham, UK > Faculty of Science > School of Computer Science
Identification Number: 10.1093/jxb/erv006
Depositing User: Eprints, Support
Date Deposited: 29 Mar 2017 11:08
Last Modified: 13 Oct 2017 01:19
URI: http://eprints.nottingham.ac.uk/id/eprint/41629

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