Identification of a Cyanine-dye labeled peptidic ligand for Y₁R and Y₄R, based upon the Neuropeptide Y C-terminal analogue, BVD-15

Liu, Mengjie and Richardson, Rachel R. and Mountford, Simon J. and Zhang, Lei and Tempone, Matheus H. and Herzog, Herbert and Holliday, Nicholas D. and Thompson, Philip E. (2016) Identification of a Cyanine-dye labeled peptidic ligand for Y₁R and Y₄R, based upon the Neuropeptide Y C-terminal analogue, BVD-15. Bioconjugate Chemistry, 27 (9). pp. 2166-2175. ISSN 1520-4812

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Abstract

Traceable truncated Neuropeptide Y (NPY) analogues with Y₁ receptor (Y₁R) affinity and selectivity are highly desirable tools in studying receptor location, regulation, and biological functions. A range of fluorescently labeled analogues of a reported Y₁R/Y₄R preferring ligand BVD-15 have been prepared and evaluated using high content imaging techniques. One peptide, [Lys²(sCy5), Arg⁴]BVD-15, was characterized as an Y₁R antagonist with a pKD of 7.2 measured by saturation analysis using fluorescent imaging. The peptide showed 8-fold lower affinity for Y₄R (pKD = 6.2) and was a partial agonist at this receptor. The suitability of [Lys²(sCy5), Arg⁴]BVD-15 for Y₁R and Y₄R competition binding experiments was also demonstrated in intact cells. The nature of the label was shown to be critical with replacement of sCy5 by the more hydrophobic Cy5.5 resulting in a switch from Y₁R antagonist to Y₁R partial agonist.

Item Type: Article
Additional Information: This document is the unedited author's version of a submitted work that was subsequently accepted for publication in the journal Bioconjugate Chemistry, Copyright © 2016 American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00376
Keywords: Neuropeptide Y, Fluorescent ligand, G protein coupled receptor, High content imaging
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1021/acs.bioconjchem.6b00376
Depositing User: Holliday, Nicholas
Date Deposited: 23 Mar 2017 09:04
Last Modified: 13 Oct 2017 01:25
URI: http://eprints.nottingham.ac.uk/id/eprint/41450

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