Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls

Hagen, Casper P. and Mieritz, Mikkel G. and Nielsen, John E. and Anand-Ivell, Ravinder and Ivell, Richard and Juul, Anders (2015) Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls. Fertility and Sterility, 103 (3). pp. 780-786. ISSN 0015-0282

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Objective: To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones.

Design: Population-based cohort study.

Setting: Not applicable.

Patient(s): Healthy peripubertal girls (n¼10) examined every 6 months; total number of examinations was 84; median (range) per girl:9 (4–10), including staging of pubertal breast development and blood samples.

Intervention(s): None.

Main Outcome Measure(s): Serum levels of INSL3, inhibin B, E2, antim€ullerian hormone, LH, and FSH (validated immunoassays), and T and androstenedione (liquid chromatography–tandem mass spectrometry).

Result(s): Serum levels of INSL3 varied considerably between girls (range, 0.01–0.27 ng/mL) and within each girl as puberty progressed; intraindividual variation, median (range) 102% (65%–143%). Insulin-like peptide 3 increased in late puberty (B1 to B4þB5); geometric mean 0.03 ng/mL to 0.15 ng/mL. Insulin-like peptide 3 levels reflected markers of large follicles (T, androstendione, inhibin B, and E2) better than markers of small follicles (antim€ullerian hormone), and INSL3 staining was localized in theca interna cells of antral follicles.

Conclusion(s): Insulin-like peptide 3 increased in late puberty, albeit inter- and intraindividual variations were substantial. Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 in girls is a unique and specific marker of theca cells surrounding antral follicles. The potential clinical use of INSL3 for evaluation of ovarian function in girls remains to be elucidated.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/743989
Keywords: INSL3, puberty, theca, girl, follicle
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Animal Sciences
Identification Number: https://doi.org/10.1016/j.fertnstert.2014.11.014
Depositing User: Anand-Ivell, Dr Ravinder
Date Deposited: 23 Oct 2017 15:24
Last Modified: 04 May 2020 17:01
URI: http://eprints.nottingham.ac.uk/id/eprint/41445

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