Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them

Ivell, Richard and Teerds, Katja and Hoffman, Gloria E (2014) Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them. Endocrinology, 155 (3). pp. 676-687. ISSN 1945-7170

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Abstract

For several decades antibodies raised against specific proteins, peptides, or peptide epitopes have proven to be versatile and very powerful tools to demonstrate molecular identity in cells and tissues. New techniques of immunohistochemistry and immunofluorescence have improved both the optical resolution of such protein identification as well as its sensitivity, particularly through the use of amplification methodology. However, this improved sensitivity has also increased the risks of false-positive and false-negative staining and thereby raised the necessity for proper and adequate controls. In this review, the authors drawonmanyyears of experience to illuminate many of the more common errors and problematic issues in immunohistochemistry, and how these may be avoided. A key factor in all of this is that techniques need to be properly documented and especially antibodies and procedures must be adequately described. Antibodies are a valuable and shared resource within the scientific community; it is essential therefore that mistakes involving antibodies and their controls are not perpetuated through inadequate reporting in the literature.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Animal Sciences
Identification Number: 10.1210/en.2013-1971
Depositing User: Anand-Ivell, Dr Ravinder
Date Deposited: 24 Oct 2017 12:24
Last Modified: 24 Oct 2017 12:27
URI: http://eprints.nottingham.ac.uk/id/eprint/41434

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