Mouse mitochondrial lipid composition is defined by age in brain and muscle

Pollard, Amelia K. and Ortori, Catharine A. and Stöger, Reinhard and Barrett, David A. and Chakrabarti, Lisa (2017) Mouse mitochondrial lipid composition is defined by age in brain and muscle. Aging, 9 (3). pp. 986-995. ISSN 1945-4589

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Abstract

Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with high resolution mass spectrometry was used to establish and compare mitochondrial lipidomes of brain and skeletal muscle from young (4-11 weeks old) and middle age (78 weeks old) healthy mice. In middle age the brain mitochondria had reduced levels of fatty acids, particularly polyunsaturated fatty acids, while skeletal muscle mitochondria had a decreased abundance of phosphatidylethanolamine, but a pronounced increase of triglyceride levels. Reduced levels of phosphatidylethanolamines are known to decrease mitochondrial membrane fluidity and are connected with accelerated ageing. In mitochondria from skeletal muscle we propose that increased age causes a metabolic shift in the conversion of diacylglycerol so that triglycerides predominate compared with phosphatidylethanolamines. This is the first time mitochondrial lipid content in normal healthy mammalian ageing brain and muscle has been catalogued in detail across all lipid classes. We identify distinct mitochondrial lipid signatures that change with age, revealing tissue-specific lipid pathways as possible targets to ameliorate ageing-related mitochondrial decline.

Item Type: Article
Keywords: mitochondria, global lipidomics, ageing, mouse, skeletal muscle, brain
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Animal Sciences
University of Nottingham, UK > Faculty of Science > School of Pharmacy
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.18632/aging.101204
Depositing User: Eprints, Support
Date Deposited: 15 Mar 2017 11:28
Last Modified: 17 May 2017 13:56
URI: http://eprints.nottingham.ac.uk/id/eprint/41327

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