Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals

Ferns, R. Bridget and Tarr, Alexander W. and Hue, Stephane and Urbanowicz, Richard A. and McClure, C. Patrick and Gilson, Richard and Ball, Jonathan K. and Nastouli, Eleni and Garson, Jeremy A. and Pillay, Deenan (2016) Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals. Virology, 492 . pp. 213-224. ISSN 0042-6822

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Abstract

HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype neutralization assays to explore the consequences of viral quasispecies evolution from pre-seroconversion to chronicity in four co-infected individuals (mean follow up 566 days). We observed that one to three founder viruses were transmitted. Relatively low viral sequence diversity, possibly related to an impaired immune response, due to HIV infection was observed in three patients. However, the fourth patient, after an early purifying selection displayed increasing E2 sequence evolution, possibly related to being on suppressive antiretroviral therapy. Viral pseudotypes generated from HCV variants showed relative resistance to neutralization by autologous plasma but not to plasma collected from later time points, confirming ongoing virus escape from antibody neutralization.

Item Type: Article
Keywords: HCV quasispecies; HIV-1 co-infection; Single genome sequencing; Founder virus; HCV pseudotype neutralization; Antiretroviral therapy
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1016/j.virol.2016.02.003
Depositing User: Eprints, Support
Date Deposited: 08 Feb 2017 14:43
Last Modified: 02 Apr 2017 06:47
URI: http://eprints.nottingham.ac.uk/id/eprint/40451

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