Evolution of complexity in the zebrafish synapse proteome

Bayes, Alex and Collins, Mark O. and Reig-Vader, Rrita and Gou, Gemma and Goulding, David and Izquierdo, Abril and Choudhary, Jyoti S. and Emes, Richard D. and Grant, Seth G.N. (2017) Evolution of complexity in the zebrafish synapse proteome. Nature Communications . ISSN 2041-1723

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Abstract

The proteome of human brain synapses is highly complex and mutated in over 130 diseases. This complexity arose from two whole genome duplications early in the vertebrate lineage. Zebrafish are used in modelling human diseases, however its synapse proteome is uncharacterised and whether the teleost-specific genome duplication (TSGD) influenced complexity is unknown. We report the characterisation of the proteomes and ultrastructure of central synapses in zebrafish and analyse the importance of the TSGD. While the TSGD increases overall synapse proteome complexity, the Post Synaptic Density (PSD) proteome of zebrafish has lower complexity than mammals. A highly conserved set of ~1000 proteins is shared across vertebrates. PSD ultrastructural features are also conserved. Lineage-specific proteome differences indicate vertebrate species evolved distinct synapse types and functions. The datasets are a resource for a wide range of studies and have important implications for the use of zebrafish in modelling human synaptic diseases.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1038/ncomms14613
Depositing User: Emes, Richard
Date Deposited: 03 Mar 2017 09:51
Last Modified: 16 Oct 2017 09:15
URI: http://eprints.nottingham.ac.uk/id/eprint/40100

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