Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Bilverstone, T.W. and Kinsmore, N.L. and Minton, Nigel P. and Kuehne, Sarah A. (2017) Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44 . pp. 51-54. ISSN 1095-8274

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Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Item Type: Article
Keywords: C. difficile; PaLoc; Binary toxin; CdtR; Virulence
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1016/j.anaerobe.2017.01.009
Depositing User: Eprints, Support
Date Deposited: 23 Jan 2017 12:32
Last Modified: 21 Apr 2017 08:41
URI: http://eprints.nottingham.ac.uk/id/eprint/39999

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