Quantitative analysis of lab-to-lab variability in Caco-2 permeability assays

Lee, Jong Bong and Zgair, Atheer and Taha, Dhiaa A. and Zang, Xiaowei and Kagan, Leonid and Kim, Tae Hwan and Kim, Min Gi and Yun, Hwi-yeol and Fischer, Peter M. and Gershkovich, Pavel (2017) Quantitative analysis of lab-to-lab variability in Caco-2 permeability assays. European Journal of Pharmaceutics and Biopharmaceutics, 114 . pp. 38-42. ISSN 1873-3441

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Abstract

In this study, Caco-2 permeability results from different laboratories were compared. Six different sets of apparent permeability coefficient (Papp) values reported in the literature were compared to experimental Papp obtained in our laboratory. The differences were assessed by determining the root mean square error (RMSE) values between the datasets, which reached levels as high as 0.581 for the training set compounds, i.e. ten compounds with known effective human permeability (Peff). The consequences of these differences in Papp for prediction of oral drug absorption were demonstrated by introducing the Papp into the absorption and pharmacokinetics simulation software application GastroPlus™ for prediction of the fraction absorbed (Fa) in humans using calibrated “user-defined permeability models”. The RMSE were calculated to assess the differences between the simulated Fa and experimental values reported in the literature. The RMSE for Fa simulated with the permeability model calibrated using experimental Papp from our laboratory was 0.128. When the calibration was performed using Papp from literature datasets, the RMSE values for Fa were higher in all cases except one. This study shows quantitative lab-to-lab variability of Caco-2 permeability results and the potential consequences this can have in the use of these results for predicting intestinal absorption of drugs.

Item Type: Article
Keywords: Drug permeability; Caco-2 permeability assay; Lab-to-lab variability; Prediction of oral absorption; GastroPlus
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1016/j.ejpb.2016.12.027
Depositing User: Eprints, Support
Date Deposited: 17 Jan 2017 09:22
Last Modified: 15 Oct 2017 15:54
URI: http://eprints.nottingham.ac.uk/id/eprint/39895

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