The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria

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Rauch, Cyril, Cherkaoui-Rbati, Mohammed, Egan, Sharon A. and Leigh, James A. (2017) The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria. BBA - Biomembranes, 1859 (2). pp. 282-288. ISSN 0005-2736

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Abstract

Background: The anionic-polyelectrolyte nature of the wall of Gram-positive bacteria has long been suspected to be involved in homeostasis of essential cations and bacterial growth. A better understanding of the coupling between the biophysics and the biology of the wall is essential to understand some key features at play in ion-homeostasis in this living system.

Methods: We consider the wall as a polyelectrolyte gel and balance the long-range electrostatic repulsion within this structure against the penalty entropy required to condense cations around wall polyelectrolytes. The resulting equations define how cations interact physically with the wall and the characteristic time required for a cation to leave the wall and enter into the bacterium to enable its usage for bacterial metabolism and growth.

Results: The model was challenged against experimental data regarding growth of Gram-positive bacteria in the presence of varying concentration of divalent ions. The model explains qualitatively and quantitatively how divalent cations interact with the wall as well as how the biophysical properties of the wall impact on bacterial growth (in particular the initiation of bacterial growth).

Conclusion: The interplay between polymer biophysics and the biology of Gram positive bacteria is defined for the first time as a new set of variables that contribute to the kinetics of bacterial growth.

General significance: Providing an understanding of how bacteria capture essential metal cations in way that does not follow usual binding laws has implications when considering the control of such organisms and their ability to survive and grow in extreme environments.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/970733
Keywords: Gram-positive bacteria; teichoic acid; cell wall; metal cations; polyelectrolytes; Manning’s Theory
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.1016/j.bbamem.2016.12.002
Depositing User: Eprints, Support
Date Deposited: 06 Dec 2016 15:04
Last Modified: 04 May 2020 19:58
URI: https://eprints.nottingham.ac.uk/id/eprint/39194

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