Human adaptation of Ebola virus during the West African outbreak

Urbanowicz, Richard A. and McClure, C. Patrick and Sakuntabhai, Anavaj and Sall, Amadou A. and Kobinger, Gary and Müller, Marcel A. and Holmes, Edward C. and Rey, Félix A. and Simon-Loriere, Etienne and Ball, Jonathan K. (2016) Human adaptation of Ebola virus during the West African outbreak. Cell, 167 (4). 1079-1087.e5. ISSN 1097-4172

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Abstract

The 2013–2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the cocirculation of several viral lineages. We hypothesized that this prolonged human circulation led to genomic changes that increased viral transmissibility in humans. We generated a synthetic glycoprotein (GP) construct based on the earliest reported isolate and introduced amino acid substitutions that defined viral lineages. Mutant GPs were used to generate a panel of pseudoviruses, which were used to infect different human and bat cell lines. These data revealed that specific amino acid substitutions in the EBOV GP have increased tropism for human cells, while reducing tropism for bat cells. Such increased infectivity may have enhanced the ability of EBOV to transmit among humans and contributed to the wide geographic distribution of some viral lineages.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Nottingham Digestive Diseases Centre
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1016/j.cell.2016.10.013
Depositing User: Eprints, Support
Date Deposited: 02 Dec 2016 12:00
Last Modified: 12 Oct 2017 21:57
URI: http://eprints.nottingham.ac.uk/id/eprint/39138

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