Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability

Leire, Emma and Amaral, Sandra P. and Louzao, Iria and Winzer, Klaus and Alexander, Cameron and Fernandez-Megia, Eduardo and Fernandez-Trillo, Francisco (2016) Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability. Biomaterials Science, 4 (6). pp. 998-1006. ISSN 2047-4830

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Abstract

Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can induce the formation of clusters in Vibrio harveyi, an opportunistic marine pathogen, in a generation dependent manner. Moreover, these cationic GATG dendrimers demonstrate an improved ability to induce cluster formation when compared to poly(N-[3-(dimethylamino)propyl]methacrylamide) [p(DMAPMAm)], a cationic linear polymer previously shown to cluster bacteria. Viability of the bacteria within the formed clusters and evaluation of quorum sensing controlled phenotypes (i.e. light production in V. harveyi) suggest that GATG dendrimers may be activating microbial responses by maintaining a high concentration of quorum sensing signals inside the clusters while increasing permeability of the microbial outer membranes. Thus, the reported GATG dendrimers constitute a valuable platform for the development of novel antimicrobial materials that can target microbial viability and/or virulence.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1039/C6BM00079G
Depositing User: Eprints, Support
Date Deposited: 01 Dec 2016 09:45
Last Modified: 18 Oct 2017 12:55
URI: http://eprints.nottingham.ac.uk/id/eprint/39101

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