Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin

Chee, Marcus Jenn Yang and Lycett, Grantley W. and Khoo, Teng-Jin and Chin, Chiew Foan (2016) Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin. Molecular Biotechnology . pp. 1-8. ISSN 1559-0305

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Abstract

Production of vanillin by bioengineering has gained popularity due to consumer demand towards vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a plant system. The VpVAN enzyme had been shown to directly convert ferulic acid and its glucoside into vanillin and its glucoside, respectively. As the ferulic acid precursor and vanillin were found to be the intermediates in the phenylpropanoid biosynthetic pathway of Capsicum species, this work serves as a proof-of-concept for vanillin production using Capsicum frutescens (C. frutescens or hot chili pepper). The cells of C. frutescens were genetically transformed with a codon optimized VpVAN gene via biolistics. Transformed explants were selected and regenerated into callus. Successful integration of the gene cassette into the plant genome was confirmed by polymerase chain reaction. High performance liquid chromatography was used to quantify the phenolic compounds detected in the callus tissues. The vanillin content of transformed calli was 0.057% compared to 0.0003% in untransformed calli.

Item Type: Article
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/s12033-016-9986-2
Keywords: vanillin; ferulic acid; vanillin synthase; VpVAN; Capsicum frutescens
Schools/Departments: University of Nottingham, Malaysia > Faculty of Science > School of Biosciences
University of Nottingham, Malaysia > Faculty of Science > School of Pharmacy
University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1007/s12033-016-9986-2
Depositing User: Eprints, Support
Date Deposited: 10 Nov 2016 14:31
Last Modified: 10 Nov 2016 14:36
URI: http://eprints.nottingham.ac.uk/id/eprint/38649

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