The role of kinetic context in apparent biased agonism at GPCRs

Klein Herenbrink, Carmen and Sykes, David A. and Donthamsetti, Prashant and Canals, Meritxell and Coudrat, Thomas and Shonberg, Jeremy and Scammells, Peter J. and Capuano, Ben and Sexton, Patrick M. and Charlton, Steven J. and Javitch, Jonathan and Christopoulos, Arthur and Lane, J. Robert (2016) The role of kinetic context in apparent biased agonism at GPCRs. Nature Communications, 7 . 10842/1-10842/14. ISSN 2041-1723

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Abstract

Biased agonism describes the ability of ligands to stabilize different conformations of a GPCR linked to distinct functional outcomes and offers the prospect of designing pathway-specific drugs that avoid on-target side effects. This mechanism is usually inferred from pharmacological data with the assumption that the confounding influences of observational (that is, assay dependent) and system (that is, cell background dependent) bias are excluded by experimental design and analysis. Here we reveal that ‘kinetic context’, as determined by ligand-binding kinetics and the temporal pattern of receptor-signalling processes, can have a profound influence on the apparent bias of a series of agonists for the dopamine D2 receptor and can even lead to reversals in the direction of bias. We propose that kinetic context must be acknowledged in the design and interpretation of studies of biased agonism.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: 10.1038/ncomms10842
Depositing User: Charlton, Steven
Date Deposited: 05 Apr 2017 07:36
Last Modified: 14 Oct 2017 16:54
URI: http://eprints.nottingham.ac.uk/id/eprint/38578

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