QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems

Zhang, Ying and Thomas, Catherine L. and Xiang, Jinxia and Long, Yan and Wang, Xiaohua and Zou, Jun and Luo, Ziliang and Ding, Guangda and Cai, Hongmei and Graham, Neil S. and Hammond, John P. and King, Graham J. and White, Philip J. and Xu, Fangsen and Broadley, Martin R. and Shi, Lei and Meng, Jinling (2016) QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems. Scientific Reports, 6 . 33113/1-33113/12. ISSN 2045-2322

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Abstract

A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average marker density which increased from 0.39 to 0.97 (0.82 SNP bin) per cM. Root and shoot traits were screened under low and ‘normal’ phosphate (Pi) supply using a ‘pouch and wick’ system, and had been screened previously in an agar based system. The P-efficient parent Ningyou7 had a shorter primary root length (PRL), greater lateral root density (LRD) and a greater shoot biomass than the P-inefficient parent Tapidor under both treatments and growth systems. Quantitative trait loci (QTL) analysis identified a total of 131 QTL, and QTL meta-analysis found four integrated QTL across the growth systems. Integration reduced the confidence interval by ~41%. QTL for root and shoot biomass were co-located on chromosome A3 and for lateral root emergence were co-located on chromosomes A4/C4 and C8/C9. There was a major QTL for LRD on chromosome C9 explaining ~18% of the phenotypic variation. QTL underlying an increased LRD may be a useful breeding target for P uptake efficiency in Brassica.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
Identification Number: https://doi.org/10.1038/srep33113
Depositing User: Eprints, Support
Date Deposited: 07 Nov 2016 13:37
Last Modified: 08 Nov 2016 20:58
URI: http://eprints.nottingham.ac.uk/id/eprint/38556

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